Mechanotransduction and
Nuclear functions

Research activities

Our research aims to understand how cells respond to changes of mechanical properties of their surrounding environment. We combine biochemistry, molecular biology and quantitative biophysics approaches to investigate the molecular mechanisms that control nuclear structure and function in response to mechanical stress. Recent projects focus on: How the nucleoskeleton regulates gene expression in vascular cells and in neuronal progenitors, and how intercellular transmission of mechanical signals regulates collective behavior within epithelium.

 


Positions are currently available for students and postdoctoral fellows with a wide range of backgrounds (cell biology, molecular biology, physics, engineering).


 

Team

Staff


Christophe Guilluy, PhD (Group Leader) christophe.guilluy@inserm.fr

Selected publications

Aureille J, Buffière-Ribot V, Harvey BE, Boyault C, Pernet L, Andersen T, Bacola G, Balland M, Fraboulet S, Van Landeghem L, Guilluy C. Nuclear envelope deformation controls cell cycle progression in response to mechanical force. EMBO Rep. 2019

Guilluy C, Osborne L, Van Landeghem L, Superfine R, Garcia-mata R and  Burridge K. Isolated nuclei adapt to force and reveal a mechanotransduction pathway within the nucleus. Nat Cell Biol. 2014 Mar 9. doi: 10.1038

Guilluy C, Swaminathan V, Garcia-Mata R, O’Brien ET, Superfine R, Burridge K. The Rho GEFs LARG and GEF-H1 regulate the mechanical response to force on integrins. Nat Cell Biol. 2011 Jun;13(6):722-7

Guilluy C, Burridge K. Nuclear mechanotransduction: forcing the nucleus to respond. Nucleus. 2015;6(1):19-22

Collins C, Guilluy C, Welch C, O’Brien ET, Hahn K, Superfine R, Burridge K, Tzima E. Localized tensional forces on PECAM-1 elicit a global mechanotransduction response via the integrin-RhoA pathway. Curr Biol. 2012 Nov 20;22(22):2087-94

Thompson WR, Guilluy C, Xie Z, Sen B, Brobst KE, Yen SS, Uzer G, Styner M, Case N, Burridge K, Rubin J.Mechanically activated Fyn utilizes mTORC2 to regulate RhoA and adipogenesis in mesenchymal stem cells. Stem Cells. 2013 Nov;31(11):2528-37

Complete list of publications


Sandrine Fraboulet, PhD (Associate Professsor) – sandrine.fraboulet@univ-grenoble-alpes.fr 

Selected publications

V. Mercier, M.H. Laporte, O. Destaing, B. Blot, C.M. Bloin, K. Pernet-Gallay, C. Chatellard, Y. Saoudi, C. Albigez-Rizo, C. Lamaze, S. Fraboulet, A. Petiot, R. Sadoul. ALG-2 interacting protein-X (Alix) is essential for clathrin-independent endocytosis and signaling. Scientific Report.  2016 (6):26986

Chassefeyre, J. Martinez-Hernández, F. Bertaso, N. Bouquier, B. Blot, M. Laporte, S. Fraboulet, Y. Couté, A. Devoy, A. Isaacs, K. Pernet-Gallay, R. Sadoul, L. Fagni, Y. Goldberg. Regulation of post- synaptic function by the dementia-related ESCRT-III subunit chmp2b. Journal of Neuroscience. 2015 18; 35(7):3155-73. Erratum in: J Neurosci. 2015 May 20; 35(20): 8035-7.

Chivet, F. Hemming, K. Pernet-Gallay, S. Fraboulet, R. Sadoul. Emerging role of neuronal exosomes in the central nervous system. Front Physiol. 2012 3:145.

Briese, B. Esmaeili, S. Fraboulet, E.C. Burt, S. Christodoulou, P.R. Towers, K.E. Davies, D.B. Sattelle. Deletion of smn-1, the Caenorhabditis elegans orthologue of the spinal muscular atrophy gene, results in locomotor  dysfunction and reduced lifespan. Hum Mol Genet. 2009 18(1):97-104.

A.L. Mahul-Mellier, F. Strappazzon, A. Petiot, C. Chatellard-Causse, S. Torch, B. Blot, K. Freeman, L. Kuhn, J. Garin, J.M. Verna, S. Fraboulet, R.Sadoul. Alix and ALG-2 are involved in TNF-R1 induced cell death. J. Biol. Chem. 2008 283(50):34954-65.

Complete list of publications


Monika Elzbieta Dolega, PhD (Research Associate) monika.dolega@univ-grenoble-alpes.fr

Selected publications

Dolega ME, Delarue M, Ingremeau F, Prost J, Delon A, Cappello G. Cell-like pressure sensors reveal increase of mechanical stress towards the core of multicellular spheroids under compression.  Nat. Commun.  2017 Jan 27;8:14056. doi: 10.1038/ncomms14056.

Dolega ME, Abeille F, Picollet-D’hahan N, Gidrol X.  Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development. Biomaterials. 2015 Jun;52:347-57. doi: 10.1016/j.biomaterials.2015.02.042. Epub 2015 Mar 3.

Dolega ME, Wagh J, Gerbaud S, Kermarrec F, Alcaraz JP, Martin DK, Gidrol X, Picollet-D’hahan. Facile bench-top fabrication of enclosed circular microchannels provides 3D confined structure for growth of prostate epithelial cells. PLoS One. 2014 Jun 19;9(6):e99416. doi: 10.1371/journal.pone.0099416. eCollection 2014.

Complete list of publications


Lydia Pernet, PhD (Engineer) lydia.pernet@univ-grenoble-alpes.fr  

Responsabilities in the lab:

– Experiments in biochemistry, molecular and cellular biology
– Cell culture line maintenance
– Development of ATACseq experiments

Professionnal training:

– Molecular biology -> qPCR
– Health & Safety à Prevention Assistant and workplace first-aider

Other tasks:

– In charge of the culture room maintenance
– Students supervision
– Purchasing manager

Pernet L, Faure V, Gilquin B, Dufour-Guérin S, Khochbin S, Vourc’h C. HDAC6-ubiquitin interaction controls the duration of HSF1 activation after heat shock. Mol. Biol. Cell. 2014, doi: 10.1091/mbc.E14-06-1032.


Marvin Brun-Cosme-bruny, PhD (Research Associate)  marvin.brun-cosme-bruny@univ-grenoble-alpes.fr

Publications : – Brun-Cosme-Bruny, M., Bertin, E., Coasne, B., Peyla, P., & Rafaï, S. (2019). Effective diffusivity of microswimmers in a crowded environment. The Journal of chemical physics150(10), 104901.Brun-Cosme-Bruny, M., Förtsch, A., Zimmermann, W., Bertin, E., Peyla, P., & Rafaï, S. (2020). Deflection of phototactic microswimmers through obstacle arrays. arXiv preprint arXiv:2005.05677.Brun-Cosme-Bruny, M., Borne, V., Faure, S., Maury, B., Peyla, P., & Rafai, S. (2019). Extreme congestion of microswimmers at a bottleneck constriction. arXiv preprint arXiv:1911.10681.
 
Investigating vimentin intermediate filaments’ response to mechanical stimulii within a cell monolayer
 
Background :
-Biophysics, Statistical physics & Hydrodynamics
-Diffusion process and crowd motion in Active matter 
-Microfluidics, Cells on chips

 

 



 

 

 

 

 

 

 

 

 

 

 

 

Student


Ivan DALYUK (PhD Student)

 mail_envelope_white_logo_0Ivan.dalyuk@univ-grenoble-alpes.fr

 

 

 

 

 

 

 


 

Sara BADAWI (PhD Student)

 mail_envelope_white_logo_0Sara.badawi@univ-grenoble-alpes.fr 

ALUMNI

Julien Aureille, PhD (Postdoctoral Researcher)

mail_envelope_white_logo_0julien.aureille@inserm.fr

 

 

 

 

Complete list of publications


Valentin Buffiere

valentin.buffiere@gmail.com  mail_envelope_white_logo_0


Ben Harvey (BSc Student)

mail_envelope_white_logo_0ben.harvey@student.manchester.ac.uk


Zeina Msheik (Master student) 

mail_envelope_white_logo_0ph.zeina.msheik@gmail.com

 

 

 

 

 

 

 

 


Nejma Belaadi, PhD

belaadi.nejma@univ-nantes.fr  unnamed

Complete list of publications


Angélique Millon Frémillon, PhD

angelique.millon@gmail.com

Complete list of publications

 

Around the Lab

Fundings & Support


 

Life in Lab


 

 

Collaborators


Martial Balland, PhD

Martial Balland, PhD

Laboratoire Interdisciplinaire de Physique 

Cell mechanics UGA – CNRS
Université Grenoble Alpes

Lab website

Rafael Garcia-Mata, PhD

Rafael Garcia-Mata, PhD

Garcia-Mata Lab

Department of Biological Sciences
University of Toledo

Lab website

Thomas Boudou, PhD

Thomas Boudou, PhD

Laboratoire Interdisciplinaire de Physique 

UGA – CNRS
Université Grenoble Alpes

Lab website

Jérémy Ségard, Artiste plasticien

Jérémy Ségard, Artiste plasticien

Lokotoro association founder

“Le développement d’une recherche pluridisciplinaire incluant l’art permet d’ouvrir des champs de production insoupçonnés dans l’imaginaire et la démocratie.”

Lab website

Laurent Muller, PhD

Laurent Muller, PhD

CNRS/UMR 7241 – INSERM U1050 
Role of Matrix Proteins in Hypoxia and Angiogenesis

CRIB, Collège de France
Stephane Germain’s Team
Paris, France

Lab website

Laurianne Van Landeghem, PhD

Laurianne Van Landeghem, PhD

Molecular biomedical Sciences department

NC State Veterinary Medicine
Raleigh, USA

Lab website

Jaap (JD) van Buul, PhD

Jaap (JD) van Buul, PhD

Department of Molecular Cell Biology
Sanquin Research and Landsteiner Laboratory

Academic Medical Center
University of Amsterdam
Amsterdam, Netherlands

Lab website

Map